| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | NT | Unverified: | ||||
| 3 | Kirkwood High School | A | Ross | Welch | Smith | Reynolds | 3:43.00 | Unverified: |
| 4 | Chaminade College Preparatory Sc | A | Carey | Daood | Fields | Max) Nottingham | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Northwest (Cedar Hill) High Scho | A | Holland | Wilson | Campbell | Grellner | 3:38.69 | Verified |
| 3 | Riverview Gardens High School | A | Abel | Carlock | Crymes | Davis | 3:35.36 | Unverified: STEAM ACADEMY Spring Invitationa |
| 4 | St. Mary's (St. Louis) High Scho | A | Green | Washington | Valentine | Strong | 3:36.49 | Verified |
| 5 | St. Louis University High School | A | 3:40.60 | Unverified: |
