| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DORSEY, Jocelyn | SO | Hannibal High School | 5:36.95 | Verified |
| 2 | SCHUERING, Olivia | SO | Quincy High School | 5:50.32 | Verified |
| 3 | MCCLELLAND, Amick | 9 | Quincy High School | 5:55.00 | Unverified: Quincy, Liberty, Palmyra Triangu |
| 4 | BONVILLIAN, Ava | 9 | Hannibal High School | 6:30.17 | Verified |
| 5 | HENNEBERRY, Kalea | SO | Mexico High School | NT | |
| 6 | JOHNSON, Mia | SO | Clark County High School | 6:37.32 | Verified |
| 7 | LITTLE, TRINITY | 9 | Clark County High School | 6:44.61 | Verified |
| 8 | WYMAN, Jordyn | 9 | Mexico High School | 6:51.10 | Verified |
