| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Quincy High School | A | Clevenger | Herring | Oliver | Bruening | 1:43.38 | Unverified: |
| 3 | Mexico High School | A | DiBlasi | Frame | Rowan | Grimes | 1:34.93 | Verified |
| 4 | Hannibal High School | A | Jaworski | Odom | Hull | Turner | 1:40.00 | Unverified: Jefferson City & Hannibal &Capit |
| 5 | Clark County High School | A | SHINN | Day | HAMNER | WHEELER | 1:45.02 | Verified |
