| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hannibal High School | A | Hull | Jurado | Whiteside | Odom | 4:10.24 | Unverified: |
| 3 | Mexico High School | A | Grimes | Vega-Onate | DiBlasi | Frame | 3:39.10 | Verified |
| 4 | Clark County High School | A | SHINN | Harper | HAMNER | WHEELER | 3:55.22 | Verified |
| 5 | Quincy High School | A | Krutmeier | Oliver | O'Hara | MacDonough | 4:12.00 | Unverified: |
