| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mexico High School | A | Turlington | Smythe | Raines | Wyman | 4:40.84 | Verified |
| 3 | Clark County High School | A | YATES | Pearl | LITTLE | WEAVER | 4:29.78 | Verified |
| 4 | Hannibal High School | A | Thompson | Groth | Karr | Dorsey | 4:32.63 | Verified |
| 5 | Quincy High School | A | Summers | Schneider | Harker | Kemner | 5:03.00 | Unverified: |
