| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hannibal High School | A | Lightle | Schisler | Ray | Septer | 9:00.83 | Verified |
| 2 | Mexico High School | A | Peuster | Frame | Grimes | Fairchild | 9:18.54 | Verified |
| 3 | Quincy High School | A | MacDonough | Leigh | Lithila | Hess | 9:45.00 | Unverified: |
| 4 | Clark County High School | A | Harper | HARDIN | BURGESS | BOWEN | 9:53.45 | Verified |
