| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOLLENDER, Brinley | SR | Clark County High School | 1:06.45 | Verified |
| 2 | NEVES, LUNDYN | SO | Clark County High School | 59.57 | Verified |
| 3 | THOMPSON, Jorie | SO | Hannibal High School | 52.46 | Verified |
| 4 | LAJOY, Jaylianna | 9 | Hannibal High School | 56.71 | Verified |
| 5 | ENGLAND, Kamrin | SO | Quincy High School | 1:00.95 | Verified |
| 6 | KEMNER, Gianna | 9 | Quincy High School | 1:11.40 | Verified |
