| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAVIS, KASSEN | 9 | Clark County High School | NT | Unverified: |
| 2 | OBERHAUS, Isaac | 9 | Bowling Green High School | 1:14.19 | Verified |
| 3 | BOWEN, GAGE | 9 | Clark County High School | 1:04.12 | Verified |
| 4 | RAY, Noah | 9 | Hannibal High School | 1:04.99 | Verified |
| 5 | LEWIS, Tyler | 9 | Bowling Green High School | 1:15.52 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KRUTMEIER, Carter | SO | Quincy High School | 1:00.89 | Verified |
| 2 | FRAME, Davontae | 9 | Mexico High School | 1:00.23 | Verified |
| 3 | WILLIAMS, Aneyas | SO | Hannibal High School | 54.05 | Verified |
| 4 | TURNER, Dorian | SO | Hannibal High School | 57.45 | Verified |
| 5 | VEGA-ONATE, Eric | 9 | Mexico High School | 1:00.79 | Verified |
| 6 | OLIVER, Nathan | 9 | Quincy High School | 1:01.20 | Verified |
