| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Quincy High School | A | Clevenger | McDade | Bruening | O'Hara | 49.43 | Unverified: |
| 3 | Clark County High School | A | SHINN | Day | WHEELER | HAMNER | 45.56 | Verified |
| 4 | Hannibal High School | A | Jaworski | Odom | Davis | Turner | 47.00 | Unverified: 62nd LickLider Invite |
