| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WENNERSTEN, Camryn | Garden State Track C | 4:43.83 | Mile PR | |
| 2 | EUDALY, MaryEllen | Cambridge Christian | 4:44.76 | 1600M PR | |
| 3 | HUMPHRIES, Samantha | Flower Mound High | 4:44.90 | 1600M PR | |
| 4 | INCE, Ali | Normal Community HS | 4:46.00 | 1600M PR | |
| 5 | DAVID, Tatum | Richland County Olne | 4:47.52 | 1600M PR | |
| 6 | KWIATKOWSKI, Katarzyna | Episcopal High Schoo | 4:47.82 | 1600M PR | |
| 7 | NOE, Paityn | Ballard High School | 4:51.76 | 1600M PR | |
| 8 | TROBER, Mya | Blue Springs South | 4:52.11 | 1600M PR | |
| 9 | TYSON, Grace | Lafayette (Wildwood) | 4:54.79 | 1600M PR | |
| 10 | BAKKER, Olivia | Blue Valley West | 4:56.82 | 1600M PR | |
| 11 | KRUGER, Ally | Liberty (Wentzville) | 4:57.19 | 1600M PR | |
| 12 | SCHIEPPE, Peyton | O'Fallon Township HS | 4:57.60 | 1600M PR | |
| 13 | DUNN, Jennalee | Joplin High School | 4:57.61 | 1600M PR | |
| 14 | RYBAK, Elena | Father McGivney Cath | 4:58.13 | 1600M PR | |
| 15 | MAZZEI, Avery | Jenks High School | 4:58.40 | 1600M PR | |
| 16 | LOWRY, Tarryn | Jenks High School | 5:00.21 | 1600M PR |
