| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAZZEI, Deborah | Jenks High School | 2:09.86 | PR | |
| 2 | HEITZIG, Becca | Lincoln Community HS | 2:09.99 | PR | |
| 3 | KELLY, Clare | Van Meter High Schoo | 2:12.11 | PR | |
| 4 | RINN, Brianna | Lincoln Southwest HS | 2:12.65 | PR | |
| 5 | CLARK, Makayla | Lees Summit West HS | 2:12.85 | PR | |
| 6 | COMER, Ahry | Pope County High Sch | 2:14.23 | PR | |
| 7 | WILMES, Elyse | Father Tolton | 2:14.38 | PR | |
| 8 | BAKER, Josie | Kirkwood High School | 2:15.65 | PR |
