| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RAY, Julia | MICDS | 5:02.40 | 1600M PR | |
| 2 | GENEL, Lilah | Parkview High School | 5:03.08 | 1600M PR | |
| 3 | CROUCH, Cami | Nerinx Hall | 5:03.70 | 1600M PR | |
| 4 | JUSTICE, Cayden | Niwot High School | 5:04.02 | 1600M PR (Alititude) | |
| 5 | OUELLETTE, Samantha | Central Magnet HS | 5:04.09 | 1600M PR | |
| 6 | LUNA, Francie | Cor Jesu Academy | 5:04.21 | 1600M PR | |
| 7 | HELMERS, Claire | Ames High School | 5:04.82 | 1600M PR | |
| 8 | MONTOYA, Emma | Los Alamos HS | 5:05.47 | 1600M PR (Altitude) | |
| 9 | TOBABEN, Kaylee | Olathe North High Sc | 5:05.52 | 1600M PR | |
| 10 | GALINDO, Madison | Bentonville High Sch | 5:06.88 | 1600M PR | |
| 11 | WILKES, Keilah | Pattonville High Sch | 5:07.28 | 1600M PR | |
| 12 | TURK, Marley | Ames High School | 5:07.70 | 1600M PR | |
| 13 | SOMMER, Lydia | Pleasant Valley HS | 5:08.06 | 1600M PR | |
| 14 | MEISER, Hannah | Civic Memorial High | 5:09.06 | 1600M PR |
