| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NELSON, Karisa | Atalanta | 4:30.10 | Mile PR | |
| 2 | TEUBEL, Alexina | Northern Iowa Track | 4:32.00 | Mile PR | |
| 3 | HERMANSSON, Hanna | Valor Track Club | 4:29.81 | Mile PR | |
| 4 | EJORE, Susan | Cascadia Elite | 4:40.99 | Mile PR | |
| 5 | WASSERMAN, Katie | HOKA NAZ Elite | 4:43.52 | Mile PR | |
| 6 | RANDALL, Jenn | Cascadia Elite | 4:33.0h | Mile PR | |
| 7 | MORRISSEY, Jamie | Atalanta | 4:32.84 | Mile PR | |
| 8 | SUGHROUE, Molly | Tracksmith ASP | 4:31.54 | Mile PR | |
| 9 | LUCKI, Alex | Toronto West Athleti | 4:35.49 | Mile PR | |
| 10 | MENGHINI, Melissa | Unattached | 4:38.62 | Mile PR | |
| 11 | CONNOR, Anna | Cascadia Elite | 4:39.95 | Mile PR | |
| 13 | FOLLETT, Katie | Elevated Performance | 4:25.48 | Mile PR | |
| 14 | MCNAMARA, Gina | Georgetown Running | 4:43.74 | Mile PR | |
| 15 | COOK, Natalie | Flower Mound High | 4:36.00 | 1600M PR | |
| 16 | WRIGHT, Yazmine | Unattached | 4:45.45 | Mile PR |
