| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HEIDESCH, Jackson | Dowling Catholic HS | 4:07.30 | 1600M PR | |
| 2 | DOWNS, Tyler | Moutain Vista HS | 4:08.39 | 1600M PR (Altitude) | |
| 3 | BETTS, Hudson | Fayetteville High Sc | 4:09.96 | 1600M PR | |
| 4 | WELCH, Dawson | Har-Ber High School | 4:10.05 | 1600M PR | |
| 5 | SHIVELY, Clay | Wichita-Trinity Acad | 4:10.08 | 1600M PR | |
| 6 | DODDA, Sahil | St. Marks | 4:10.27 | 1600M PR | |
| 7 | NEWKIRK, Tanner | Hayden Catholic HS | 4:10.88 | 1600M PR | |
| 8 | WATTS, Ryan | Edwardsville HS | 4:11.16 | 1600M PR | |
| 9 | TALLEY, Tanner | New Covenant Academy | 4:11.29 | 1600M PR | |
| 10 | KRASNOFF , Charlie | Ladue Horton Watkins | 4:11.54 | 1600M PR | |
| 11 | HAUSER, Andrew | Rock Bridge High Sch | 4:11.72 | 1600M PR | |
| 12 | JOKERST, Weston | Rock Bridge High Sch | 4:11.88 | 1600M PR | |
| 13 | FREINER, Tyler | Timberland High Scho | 4:11.92 | 1600M PR | |
| 14 | CAMPBELL, Hobbs | Joplin High School | 4:12.19 | 1600M PR | |
| 15 | SANCHEZ, Angel | Diamond Hill Jarvis | 4:12.59 | 1600M PR |
