| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WALKER, Mae | 8 | Gentry Middle School | 5:18.00 | |
| 2 | BAKER, Maggie | 7 | Nashville Youth Athl | 5:27.00 | |
| 3 | STANLEY, Emma | 7 | XLR8 Track Club | 5:30.00 | |
| 4 | MESSNER, Ella | 6 | Academie Lafayette | 5:30.00 | |
| 5 | MCKENZIE, Mary | 8 | St. Louis Blazers | 5:34.00 | |
| 6 | AMANN, Savannah | 8 | St. Louis Blazers | 5:40.00 | |
| 7 | COX, Kinslee | 6 | Nashville Youth Athl | 5:44.00 | |
| 8 | KRUEGER, Brianna | 7 | Immanuel Lutheran Sc | 5:47.00 | |
| 9 | COLE, Olivia | 8 | Festus Middle School | 5:47.00 | |
| 10 | JENKERSON, Kate | 7 | Farmington MS | 5:52.00 | |
| 11 | ALLEN, Brailynn | 7 | Farmington MS | 5:56.00 | |
| 12 | HAYNES, Jocelyn | 8 | Troy | 5:56.00 | |
| 13 | GROENEWALD, Marli | 6 | St. Louis Blazers | 5:57.00 | |
| 14 | DUNHAM, Ruth | 7 | Pella Middle School | 5:57.00 | |
| 15 | MEEK, Haley | 8 | St. Louis Blazers | 5:58.00 | |
| 16 | MIELE, Allison | 8 | St. Louis Blazers | 5:58.00 | |
| 17 | STANN, Kailyn | 8 | St. Justin Martyr | 5:58.00 | |
| 18 | APPLEGATE, Frances | 7 | MICDS | 6:03.00 | |
| 19 | SHIPLEY, Madilynn | 7 | Farmington MS | 5:53.00 |
