| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rockhurst High School | A | Drouin | Lewis | Thorpe | Willis | NT | Unverified: |
| 2 | Raymore-Peculiar High School | A | NT | Unverified: | ||||
| 3 | Truman High School | A | Tapusoa | Chapin | Shepard | Orth | 1:03.36 | Verified |
| 4 | Grain Valley High School | A | Perkins | Nelson | McAnerney | Williams | 53.46 | Verified |
| 5 | Blue Springs South High School | A | Murphy | Sauni | Richberg | Newland | 55.00 | Unverified: |
| 6 | Liberty High School | A | Bindel | Lewis | Ndifor | Henson | NT | Unverified: |
| 7 | North Kansas City High School | A | Peterson | Baehr | Hill | Adebawore | NT | Unverified: |
