| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Notre Dame de Sion High School | A | Saxton | Renners | Ford | Burdge | NT | Unverified: |
| 2 | Lee's Summit West High School | A | Johnson | Jones | Carroll | Amezcua | 55.00 | Unverified: |
| 3 | Grain Valley High School | A | Huber | Sears | Sanchez | Moore | 1:01.86 | Verified |
| 4 | North Kansas City High School | A | Williams | Perdomo | Sage | Foley | NT | Unverified: |
| 5 | Truman High School | A | McIntyre | Rush | Stone | Palelei | 1:00.23 | Verified |
| 6 | Blue Springs South High School | A | NT | Unverified: |
