| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Notre Dame de Sion High School | A | Traylor | Shelton | Lind | Deacan | 4:51.77 | Verified |
| 2 | Cameron High School | A | Garr | Wenck | McVicker | Robinson | 4:18.32 | Verified |
| 3 | Raymore-Peculiar High School | A | Clark | Simmons | Mitchell | Wade | 4:11.74 | Verified |
| 4 | Lee's Summit West High School | A | Farrington | Broesder | Scott | Clark | 3:56.77 | Verified |
| 5 | Grain Valley High School | A | Huffman | Ogle | Rogers | Fisher | 4:10.54 | Verified |
| 6 | Ruskin High School | A | Taylor | Arceneaux | Ross | Fontleroy | 4:16.30 | Unverified: |
| 7 | Fort Osage High School | A | Franks | Chiesi | Rinacke | Wilson | DNS | Verified |
