| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WILLIAMS, Aaliyah | 9 | Blue Springs High School | 1:00.11 | Verified |
| 4 | STONE, Samantha | SO | Truman High School | 59.47 | Verified |
| 5 | HARRIS, Aaliyah | 9 | Blue Springs High School | 1:00.07 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | VIGIL, Madison | SR | Fort Osage High School | 58.05 | Verified |
| 3 | FULLER, Isabelle | SO | Fort Osage High School | 56.58 | Verified |
| 4 | WILSON, Nevaeh | SO | Fort Osage High School | 53.90 | Verified |
| 5 | WREN, Kate | SO | Notre Dame de Sion High School | 54.71 | Verified |
| 6 | SIPES, Kelise | 9 | Blue Springs High School | 56.76 | Verified |
| 7 | PAYNE, Zoe | SO | North Kansas City High School | 59.06 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HERL, Jayden | 9 | Truman High School | 53.35 | Verified |
| 2 | VERDUGHT, Morgan | SR | North Kansas City High School | 50.06 | Verified |
| 3 | WADE, Khyla | JR | Raymore-Peculiar High School | 49.19 | Verified |
| 4 | PARKER, LaNicia | 9 | North Kansas City High School | 46.50 | Verified |
| 5 | OGLE, Emma Jane | SO | Grain Valley High School | 49.06 | Verified |
| 6 | LYON, Liberty | JR | Lee's Summit West High School | 49.59 | Verified |
| 7 | WENCK, Claire | JR | Cameron High School | 52.44 | Verified |
| 8 | BEELMAN, Grace | SR | Notre Dame de Sion High School | 53.73 | Verified |
