| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Columbia Independent | A | Craver | Fullerton | Rush | Meyer | 5:45.00 | |
| 3 | Jefferson | A | Ireland | London | Willenberg | Weber | 5:01.00 | |
| 4 | Gentry | A | Fedenia | Walker | Rodgers | Metz | 4:33.00 | |
| 5 | Gentry | B | Reid | Tiemeyer | Appleton | Ao | 5:00.00 | |
| 6 | Jefferson | B | Bannister | Schoeneberg | Eatherton | Goodrich | 5:01.00 |
