| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glen Carbon (Father McGivney | A | Holzinger | Joellenbeck | Moore | Diest | 50.00 | |
| 3 | Edwardsville (Metro-East Lut | A | Wren | Downs | Jose | Butler | 47.00 | |
| 4 | Alton (Marquette) | A | Hamberg | Allen | Hudson | Redd | 47.50 |
