| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUSTLER, Raina | SO | Alton (Marquette) | 39' 5.50" | |
| 2 | WINDORFF, Sophia | FR | Glen Carbon (Father McGivney | 45' 0" | |
| 3 | WRIGHT, Kathren | FR | Edwardsville (Metro-East Lut | 55' 4.50" | |
| 4 | KOCH, Morgan | SO | Edwardsville (Metro-East Lut | 63' 6.50" | |
| 5 | NOLTE, Madisyen | FR | Centralia (Christ Our Rock L | 67' 2" | |
| 6 | WHITE, Semaj | FR | Edwardsville (Metro-East Lut | 77' 6.25" | |
| 7 | FRENCH, Bryanna | SR | Centralia (Christ Our Rock L | 82' 9.25" |
