| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Alton (Marquette) | A | Rae | Wells | Sanders | Dotson | 58.50 | |
| 3 | Glen Carbon (Father McGivney | A | Hylla | Beltramea | Harris | Gilbertson | 56.00 | |
| 4 | Edwardsville (Metro-East Lut | A | Hughes | Koch | Rainey | Sherfy | 56.00 |
