| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WILKINSON, Makenna | SR | Bunker Hill | NT | |
| 3 | LEITNER, Emma | JR | Edwardsville (Metro-East Lut | 36.74 | |
| 4 | AMANSHA, Aizhan | SR | Bunker Hill | 40.04 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCLEOD, Isabelle | FR | Edwardsville (Metro-East Lut | 35.14 | |
| 2 | SIMONS, Kambria | SO | Alton (Marquette) | 28.50 | |
| 3 | RANGE, Mia | FR | Glen Carbon (Father McGivney | 27.56 | |
| 4 | HENTRICH, Sammy | JR | Alton (Marquette) | 27.84 | |
| 5 | BELTRAMEA, Amelia | SR | Glen Carbon (Father McGivney | 30.60 |
