| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RYBAK, Elena | FR | Glen Carbon (Father McGivney | 4:58.00 | |
| 2 | HATLEY, Kaitlyn | SO | Glen Carbon (Father McGivney | 5:20.00 | |
| 3 | MCKOWEN, Sarah | SO | Centralia (Christ Our Rock L | 5:47.40 | |
| 4 | JOHNSON, Katie | SO | Alton (Marquette) | 6:05.88 | |
| 5 | BRAMMEIER, Rylee | FR | Centralia (Christ Our Rock L | 6:38.30 | |
| 6 | BURRIS, Lillian | FR | Bunker Hill | 7:15.72 | |
| 7 | SCOTT, Abby | SR | Bunker Hill | 8:01.30 |
