| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MEYER, Abigail | JR | Centralia (Christ Our Rock L | 1:11.24 | |
| 3 | GILBERTSON, Lilly | FR | Glen Carbon (Father McGivney | 1:02.60 | |
| 4 | CUMMINS, Jane | FR | Glen Carbon (Father McGivney | 1:08.80 | |
| 5 | ECCLES, Cassidy | FR | Alton (Marquette) | 1:15.02 |
