| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Glen Carbon (Father McGivney | A | Holzinger | Joellenbeck | Joellenbeck | Diest | 1:50.00 | |
| 3 | Edwardsville (Metro-East Lut | A | Wren | Downs | Jose | Kohlmiller | DQ | |
| 4 | Alton (Marquette) | A | Hamberg | Allen | Acklin | Sykes | 1:45.50 |
