| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Viburnum High School | A | Hardy | Zundell | Murphy | Hubbs | 1:47.34 | Verified |
| 3 | Cuba High School | A | Hunter | Nelson | Hinojosa | Volner | 1:42.33 | Verified |
| 4 | Steelville High School | A | Harris | Killeen | Brown | Brown | 1:39.78 | Verified |
| 5 | Vienna High School | A | Magner | George | Kilmer | Steffen | 1:39.80 | Verified |
| 6 | Bourbon High School | A | Reeves | Politte | Pietraschke | Reeves | 1:44.09 | Verified |
| 7 | Belle High School | A | Smith | Gromowski | Bailey | Drewel | 1:47.71 | Verified |
