| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cuba High School | A | Dong | Moreland | Black | Deluna | 2:12.99 | Verified |
| 3 | Steelville High School | A | Kelly | Kreitner | Perkins | Evans | 2:02.32 | Verified |
| 4 | Vienna High School | A | Schulte | Novak | Schell | Reeves | 1:58.57 | Verified |
| 5 | Bourbon High School | A | Clonts | Poole | Gebhart | Enke | 2:01.21 | Verified |
| 6 | Viburnum High School | A | Pyatt | Halbert | Barker | Wigger | 2:05.05 | Verified |
