| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Belle High School | A | Smith | McDaniel | Rose | Doss | 4:06.82 | Unverified: St. James Invitational |
| 3 | Vienna High School | A | Steffen | Kilmer | Wilkinson | Casey | 4:03.99 | Verified |
| 4 | Steelville High School | A | Brown | Harris | Killeen | Settles | 3:38.48 | Verified |
| 5 | Cuba High School | A | Dake | Hunter | Nelson | Volner | 3:59.24 | Verified |
| 6 | Bourbon High School | A | Reeves | Gibbs | Pietraschke | Reeves | 4:05.49 | Verified |
