| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bourbon High School | A | Clonts | Gebhart | Poole | Enke | 4:54.55 | Verified |
| 4 | Vienna High School | A | Weeks | Schulte | Schell | Reeves | 4:30.77 | Verified |
| 5 | Steelville High School | A | Diaz | Kreitner | Pryor | Evans | 4:37.21 | Verified |
| 6 | Cuba High School | A | Deluna | Ijames | Moreland | Black | 5:08.69 | Verified |
