| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | JR | Steelville High School | 2:02.76 | Verified |
| 2 | WILKINSON, Duncan | SO | Vienna High School | 2:18.06 | Verified |
| 3 | CROCKER, Vincent | 9 | Steelville High School | 2:19.07 | Verified |
| 4 | HENKE, Dustin | SR | Cuba High School | 2:26.05 | Verified |
| 5 | MORELAND, Austin | SO | Steelville High School | 2:37.65 | Verified |
| 6 | MURPHY, Michael | 9 | Viburnum High School | 2:40.92 | Verified |
| 7 | ZUNDELL, Kameron | 9 | Viburnum High School | 2:40.95 | Verified |
| 8 | FINNEGAN, Barrett | 9 | Belle High School | 2:44.30 | Verified |
| 9 | MASSEY, John | JR | Cuba High School | 3:07.50 | Verified |
