| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Courtney | JR | Steelville High School | 2:40.00 | Verified |
| 2 | TURNBOUGH, Audrey | SO | Steelville High School | 2:43.79 | Verified |
| 3 | REEVES, Aubrey | 9 | Vienna High School | 2:46.43 | Verified |
| 4 | COLLINSON, Aleah | SO | Steelville High School | 2:51.66 | Verified |
| 5 | IJAMES, Izzabelle | SO | Cuba High School | 2:51.67 | Verified |
| 6 | CONAWAY, Jorja | SO | Viburnum High School | 2:57.11 | Verified |
| 7 | NOVAK, Madeleine | JR | Vienna High School | 3:13.00 | Verified |
| 8 | MCDANIEL, Tajel | 9 | Belle High School | 3:14.35 | Verified |
| 9 | WOOLS, Kailey | 9 | Cuba High School | 3:20.75 | Verified |
| 10 | PETERSON, April | 9 | Viburnum High School | 3:24.43 | Verified |
| 11 | DICKENS, Kaitlin | JR | Cuba High School | 3:35.63 | Verified |
| 12 | WIEBERG, Claudia | SO | Vienna High School | NT | Unverified: |
