| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | KOCH, Olivia | JR | Belle High School | NT | Unverified: |
| 4 | HIX, Hannah | 9 | Bourbon High School | 1:06.74 | Verified |
| 5 | HART, Ari | SO | Cuba High School | 1:22.35 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BARTLE, Raylee | 9 | Bourbon High School | 1:02.92 | Verified |
| 3 | WIGGER, Natalie | 9 | Viburnum High School | 55.24 | Verified |
| 4 | PERKINS, Naomi | SR | Steelville High School | 53.90 | Verified |
| 5 | DAVIS, Lauren | SR | Steelville High School | 54.28 | Unverified: SBU HS Invitational Class 1, 2, |
| 6 | EVANS, Norah | SO | Steelville High School | 1:00.65 | Verified |
| 7 | MARTELLARO, Rachel | 9 | Cuba High School | 1:04.08 | Verified |
