| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | FINNEGAN, Barrett | 9 | Belle High School | 1:15.13 | Verified |
| 3 | ZUNDELL, Kameron | 9 | Viburnum High School | 1:07.81 | Verified |
| 4 | WELLS, Riordan | SR | Viburnum High School | 1:06.38 | Verified |
| 5 | SCOTT, Charles | SR | Cuba High School | 1:06.49 | Verified |
| 6 | CARNES, Landon | 9 | Vienna High School | 1:09.00 | Unverified: Linn |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GIBBS, Keaton | 9 | Bourbon High School | 1:01.22 | Verified |
| 2 | HINOJOSA, Houston | JR | Cuba High School | 57.07 | Verified |
| 3 | HARRIS, Wyatt | JR | Steelville High School | 55.43 | Verified |
| 4 | DIAZ, Conner | JR | Steelville High School | 53.24 | Verified |
| 5 | BROWN, Toby | SR | Steelville High School | 54.65 | Verified |
| 6 | REEVES, Zackary | SO | Bourbon High School | 56.94 | Verified |
| 7 | HUNTER, Landon | 9 | Cuba High School | 1:00.05 | Verified |
| 8 | MURPHY, Michael | 9 | Viburnum High School | 1:02.10 | Verified |
