| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CONAWAY, Jorja | SO | Viburnum High School | NT | Unverified: |
| 3 | GRIFFIN, Aliyah | SO | Vienna High School | 1:20.20 | Unverified: Linn |
| 4 | KLOEPPEL, Ava | 9 | Vienna High School | 1:15.00 | Unverified: Vienna |
| 5 | BUSSELL, Allyson | JR | Cuba High School | 1:18.85 | Verified |
| 6 | SOPPELAND, Tanya | 9 | Cuba High School | 1:30.07 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BLACK, Rylee | SO | Cuba High School | 1:09.85 | Verified |
| 2 | ENKE, Marisa | 9 | Bourbon High School | 1:09.42 | Verified |
| 3 | BARKER, Alexus | SR | Viburnum High School | 1:08.05 | Verified |
| 4 | PRYOR, Daylan | JR | Steelville High School | 1:05.14 | Verified |
| 5 | WEEKS, Madison | 9 | Vienna High School | 1:06.35 | Verified |
| 6 | WIGGER, Natalie | 9 | Viburnum High School | 1:09.16 | Verified |
| 7 | CLONTS, Cierra | SO | Bourbon High School | 1:09.78 | Verified |
| 8 | GEBHART, Mia | SO | Bourbon High School | 1:12.49 | Verified |
