| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vienna High School | A | Zimmer | Jones | Cecchinato | Rollins | 55.07 | Verified |
| 4 | Cuba High School | A | Dake | Hinojosa | Volner | Nelson | 48.37 | Unverified: St James |
| 5 | Steelville High School | A | Settles | Killeen | Pounds | Brown | 48.40 | Verified |
| 6 | Belle High School | A | Butler | Gromowski | Drewel | Smith | 55.94 | Verified |
