| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Deluna | Dong | Moreland | Martellaro | 58.64 | Verified |
| 4 | Steelville High School | A | Bailey | Woodall | Evans | Kelly | 55.61 | Verified |
| 5 | Vienna High School | A | Novak | Rosentreter | Schell | Schulte | 56.99 | Verified |
