| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles High School | A | Mbroh | Chang | Murphy | Calmese | 1:37.99 | Verified |
| 3 | Francis Howell High School | A | 1:32.50 | Verified | ||||
| 4 | Ritenour High School | A | 1:28.28 | Verified | ||||
| 5 | Timberland High School | A | Hester | Schaefer | Ervin | Whitehead | 1:31.99 | Verified |
| 6 | Ft. Zumwalt West High School | A | St. Jean | Welsh | Green | Copelin | 1:34.00 | Unverified: |
| 7 | Francis Howell North High School | A | Minter | Eiland | Stojanovic | Allen | 1:40.53 | Verified |
