| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Wampler | Welty | Lee | Hockman | 8:17.42 | Verified |
| 2 | Ft. Zumwalt West High School | A | Rider | Jones | Jones | Brown | 8:25.00 | Unverified: |
| 3 | St. Charles High School | A | Maples | Wotawa | Wright | Bland | 8:30.39 | Verified |
| 4 | Francis Howell High School | A | 8:43.70 | Verified | ||||
| 5 | Francis Howell North High School | A | Alexander | Brewer | Jefferson | McGuire | 8:55.77 | Verified |
