| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | TRANKLER, Isabella | 9 | Francis Howell High School | 30.74 | Verified |
| 4 | KINNER, Taylor | SR | St. Charles High School | 30.23 | Unverified: Troy Trojan Varsity Invitational |
| 5 | SAWVELL, Kelsey | 9 | Ft. Zumwalt West High School | 30.34 | Verified |
| 6 | LAFEBER, Grace | SR | Ft. Zumwalt West High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHANCE, Lauren | SO | Francis Howell North High School | 28.85 | Verified |
| 2 | BUSATERI, Breanna | SR | Timberland High School | 28.48 | Verified |
| 3 | UTLEY, Sheridan | SO | Timberland High School | 27.83 | Verified |
| 4 | JONES, Amani | SR | Francis Howell North High School | 25.52 | Verified |
| 5 | WILLIAMS, Zora | SR | Francis Howell High School | 26.89 | Verified |
| 6 | LIGHTBODY, Azea | JR | Timberland High School | 27.85 | Unverified: |
| 7 | WASHINGTON, Madison | 9 | Ft. Zumwalt West High School | 28.80 | Verified |
| 8 | NELSON, Victoria | 9 | Francis Howell High School | 29.82 | Verified |
