| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | JR | Rock Bridge High School | 2:14.18 | Verified |
| 2 | PARSONS, Grace | JR | Rock Bridge High School | 2:22.62 | Verified |
| 3 | SHERWOOD, Ashlyn | 9 | Francis Howell High School | 2:27.86 | Verified |
| 4 | SHINABERY, Paige | SO | Francis Howell High School | 2:30.38 | Verified |
| 5 | O'KEEFFE, Atty | 9 | St. Charles High School | 2:32.56 | Verified |
| 6 | STEUBER, Maci | SR | Rock Bridge High School | 2:33.53 | Verified |
| 7 | MAUPIN, Sophia | SO | Ft. Zumwalt West High School | 2:37.16 | Verified |
| 8 | RAMADAN, Jenna | SR | Timberland High School | 2:37.19 | Verified |
| 9 | MCGRATH, Katherine | 9 | Ft. Zumwalt West High School | 2:43.02 | Verified |
| 10 | MCDANIEL, Karsyn | 9 | Ft. Zumwalt West High School | 2:49.45 | Verified |
| 11 | MATTINGLY, Avery | SO | Francis Howell High School | 2:49.72 | Verified |
| 12 | RAYBURN, Jonni | SR | St. Charles High School | 3:03.13 | Verified |
| 13 | GREEN, Melinda | 9 | St. Charles High School | NT | Unverified: |
