| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WARNER, James | SO | St. Charles High School | 47.82 | Verified |
| 3 | BOURGEADE, Luc | JR | St. Charles High School | 45.75 | Verified |
| 4 | DOWDY, Gilbren | JR | Francis Howell North High School | 44.59 | Verified |
| 5 | MILAM, Ethan | JR | Francis Howell High School | 45.72 | Verified |
| 6 | MINTER, Jordan | JR | Francis Howell North High School | 47.34 | Verified |
| 7 | ENGELHARD, Jack | SO | Francis Howell North High School | 48.18 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, Darius | SO | Ft. Zumwalt West High School | 44.24 | Verified |
| 2 | GARAVAGLIA, Lucius | JR | Ft. Zumwalt West High School | 43.56 | Unverified: |
| 3 | HART, Cameron | SR | Ft. Zumwalt West High School | 42.16 | Verified |
| 4 | RIGGINS, Vashon | SR | Timberland High School | 40.42 | Verified |
| 5 | DEAL, Joshua | JR | Francis Howell High School | 42.04 | Verified |
| 6 | NENNERT, Charles | SR | St. Charles High School | 42.39 | Verified |
| 7 | PEARSON, Jaylen | JR | Francis Howell High School | 44.00 | Unverified: |
| 8 | WILLIS, Andrew | JR | Timberland High School | 44.57 | Verified |
