| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | SIMON, Alise | SR | Francis Howell North High School | 1:12.02 | Verified |
| 4 | ST. JEAN, Gabriella | SR | Ft. Zumwalt West High School | 1:07.64 | Verified |
| 5 | NOVAK, Ali | JR | Francis Howell High School | 1:10.20 | Verified |
| 6 | NOTTMEYER, Ella | 9 | St. Charles High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REICHART, Brigid | SO | Francis Howell High School | 1:07.15 | Verified |
| 2 | COZZONI, Alyssa | SR | Timberland High School | 1:06.04 | Verified |
| 3 | RIDER, Lyla | JR | Ft. Zumwalt West High School | 1:04.12 | Verified |
| 4 | WILLIAMS, Zora | SR | Francis Howell High School | 58.81 | Verified |
| 5 | BUSATERI, Breanna | SR | Timberland High School | 1:01.63 | Verified |
| 6 | ANDERSON, Oakley | 9 | Ft. Zumwalt West High School | 1:05.14 | Verified |
| 7 | OELLERMANN, Mia | SR | St. Charles High School | 1:07.00 | Unverified: Winfield HS Early Invitational |
| 8 | KUZARA, Alyssa | SR | Timberland High School | 1:07.55 | Verified |
