| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ft. Zumwalt West High School | A | Eley | Carr | Green | Petty | 45.52 | Verified |
| 3 | Timberland High School | A | Hester | Schaefer | Ervin | Whitehead | 44.48 | Verified |
| 4 | Ritenour High School | A | 43.58 | Verified | ||||
| 5 | Francis Howell High School | A | 43.62 | Verified | ||||
| 6 | Francis Howell North High School | A | McCrary | Burris | Stojanovic | Allen | NT | Verified |
