| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ft. Zumwalt West High School | A | Loggins | Walden | Anderson | Lafeber | 56.32 | Verified |
| 3 | Francis Howell High School | A | 52.74 | Verified | ||||
| 4 | Timberland High School | A | Tucker | Lightbody | Utley | Adams | 50.75 | Verified |
| 5 | Francis Howell North High School | A | Yauna Fullenwider | Jones | Chance | Ausbury | 52.42 | Verified |
| 6 | St. Charles High School | A | Kinner | Hollrah | Klimas | Long | 54.65 | Verified |
