| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Northwest (Cedar Hill) High Scho | A | Robinson | Sullivan | Brandt | Bounds | 1:43.25 | Verified |
| 3 | Francis Howell North High School | A | Eiland | Lin | Fifer | Vob Orb | DQ | Verified |
| 4 | Ft. Zumwalt West High School | A | Carr | Smith | Dreyer | Petty | 1:37.00 | Verified |
| 5 | Christian High School | A | Connell | Schroer | States | Tucker | 1:38.00 | Verified |
| 6 | Ft. Zumwalt East High School | A | James | Franklin | Rudd | Williams | DQ | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Colyer | Goellner | Guerrero | Hunsperger | 1:36.50 | Verified |
| 2 | Ft. Zumwalt South High School | A | Raife | Powell | Bates | Dewitt | 1:36.28 | Verified |
| 3 | Timberland High School | A | Schaefer | Whitehead | Hester | Matlick | 1:34.06 | Verified |
| 4 | Ft. Zumwalt North High School | A | Bredenkamp | Weddington | Lee | Reckel | 1:32.65 | Verified |
| 5 | Francis Howell Central High Scho | A | Brown | Humphries | Thomas | Washington | 1:33.81 | Verified |
| 6 | Troy Buchanan High School | A | Giacopelli | Ryan | Hall | Benhardt | 1:34.43 | Verified |
| 7 | Holt High School | A | Mozee | Deanda | Hartweck | Adams | 1:36.32 | Verified |
| 8 | Warrenton High School | A | Meine | Bristol | Reel | Evans | 1:36.50 | Verified |
