| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell North High School | A | Huff | Huff | Bland | StJohn | 2:12.92 | Verified |
| 4 | Warrenton High School | A | Schleuter | Marschel | Love | Rhama | 1:57.10 | Verified |
| 5 | Christian High School | A | Cunningham | Phillips | Gilot | Malone | 2:07.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | 1:56.62 | Verified | ||||
| 2 | Ft. Zumwalt West High School | A | Anderson | Mueller | St. Jean | Rider | 1:54.21 | Verified |
| 3 | Ft. Zumwalt South High School | A | Moore | Johnson | Clark | Burnett | 1:51.45 | Verified |
| 4 | Troy Buchanan High School | A | Crouch | Hunolt | Starkey | Robinson | 1:51.30 | Verified |
| 5 | Timberland High School | A | Utley | Lightbody | Lewis | Kuzara | 1:51.43 | Verified |
| 6 | Francis Howell Central High Scho | A | Anthony | Sanders | Mabray | Johnson | 1:52.03 | Verified |
| 7 | Ft. Zumwalt North High School | A | Ramadan | Michel | Abdelkarim | Hamilton | 1:56.53 | Verified |
