| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Christian High School | A | Steimel | Corwin | Showalter | McNeil | 5:14.31 | Verified |
| 4 | Francis Howell Central High Scho | A | Halterman | McDevitt | Myers | Brower | NT | Verified |
| 5 | Warrenton High School | A | Marschel | Howard | Rhama | Marschel | NT | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ft. Zumwalt South High School | A | Brauch | Morrison | Harper | Whitten | 4:38.40 | Verified |
| 3 | Troy Buchanan High School | A | Cook | Getz | Ellison | Waechter | 4:33.95 | Verified |
| 4 | St. Charles West High School | A | Prost | Zeier | Myers | Jackson | 4:20.62 | Verified |
| 5 | Ft. Zumwalt West High School | A | Anderson | Mueller | Maupin | Rider | 4:24.02 | Verified |
| 6 | Northwest (Cedar Hill) High Scho | A | 4:37.78 | Verified | ||||
| 7 | Ft. Zumwalt North High School | A | Norman | Gatewood | Maier | Ebert | 4:44.29 | Verified |
