| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FORNESS, Lauren | Ft. Zumwalt North High School | NT | ||
| 4 | DUEKER, Kinsey | SR | Troy Buchanan High School | NT | Unverified: |
| 5 | FIERRO RUBIO, Leslyee | Ft. Zumwalt North High School | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BLAND, McKenzie | 9 | Francis Howell North High School | 34.16 | Verified |
| 2 | PHILLIPS, Alayna | JR | Christian High School | 32.23 | Verified |
| 3 | HACKMANN, Abigail | JR | Warrenton High School | 31.04 | Verified |
| 4 | DUNLAP, Cadence | SO | Ft. Zumwalt North High School | 30.38 | Verified |
| 5 | OVERKAMP, Kylie | SO | Warrenton High School | 30.50 | Unverified: |
| 6 | ST. JEAN, Gabriella | SR | Ft. Zumwalt West High School | 31.06 | Verified |
| 7 | SPIVEY, Nylah | JR | Ft. Zumwalt East High School | 32.84 | Verified |
| 8 | STEIMEL, Elle | 9 | Christian High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERTANI, Kayla | SR | Northwest (Cedar Hill) High Scho | 29.56 | Unverified: Kirkwood Early Bird |
| 2 | ROWOLD, Madeline | SR | Ft. Zumwalt East High School | 29.40 | Verified |
| 3 | CHANCE, Lauren | SO | Francis Howell North High School | 28.97 | Verified |
| 4 | UTLEY, Sheridan | SO | Timberland High School | 28.79 | Verified |
| 5 | BOXDORFER, Aaliyah | JR | St. Charles West High School | 28.86 | Verified |
| 6 | BRANTLEY, Rickeena | SR | Francis Howell Central High Scho | NT | |
| 7 | SHIVERS, Jillian | SR | St. Charles West High School | 29.50 | Verified |
| 8 | MOSHER, Adison | SR | Holt High School | 30.02 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RAMADAN, Layaly | SR | Ft. Zumwalt North High School | 28.73 | Verified |
| 2 | CLARK, Inaiah | SO | Ft. Zumwalt South High School | 28.10 | Verified |
| 3 | BOBEEN, Breana | SO | Troy Buchanan High School | 27.51 | Verified |
| 4 | BURNETT, Nia | SR | Ft. Zumwalt South High School | 27.35 | Verified |
| 5 | ANTHONY, Tijaria | 9 | Francis Howell Central High Scho | 27.44 | Verified |
| 6 | LIGHTBODY, Azea | JR | Timberland High School | 28.02 | Unverified: |
| 7 | BROWN, Samantha | SR | Northwest (Cedar Hill) High Scho | 28.20 | Unverified: Henle Holmes |
| 8 | RIDER, Lyla | JR | Ft. Zumwalt West High School | 28.75 | Verified |
